KIT COMPONENTS
peptone, beef extract, hemin, yeast, SPS, cysteine hydrochloride, autoclaving

SPECIMEN COLLECTION
Blood or ascites, sterile operation, can inject the sample in the bottle directly, no need special operate.

PROCEDURE
1. Check if the bottle is contaminated or broken.
2. Prepare and label the appropriate blood culture bottle
3. Do not unscrew the cap. Remove the plastic top of the screw cap on the blood culture bottle.
4. Disinfect the visible part of the rubber plug with iodine or ethyl alcohol (70%) and allow drying.
5. Collect approximately 5-10 ml of adult patients' blood or 1-3 ml of infant patients' blood
6. Inject blood, ascites, or cerebrospinal fluid into the culture bottle, after being collected with aseptic operations. Then disinfect the rubber plug again and replace the plastic top.
7. After the inoculation, lean the bottle several times, and incubate it, erectly at 35-37 ºC for 24 hours.

INTERPRETATION OF RESULT
1. Report the positive results if turbidity in the culture broth, hemolysis of blood corpuscles, air bubbles, or colonies are observed. Carry out susceptibility tests and strain identification on positive samples.
2. Soak agar state into broth every day and keep on incubating the bottle erectly if those phenomena do not occur, until the 7th day.
3. If the abovementioned phenomena still do not occur after 7 days of culturing, report a negative result after confirming with microscopy and blind passage.